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Metallosphaera sedula DSM 5348

Archaeon

Synonyms: ATCC 51363, DSM 5348

88: SULFOLOBUS MEDIUM

Modifications: Omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium.


Growth observed:

Growth conditions: 3-7 days 65 °C